黃幸宜副教授 Dr. Hsing-I Huang
Current Position: Assistant Professor, Department of Medical Biotechnology and Labroatory Sciences, Chang Gung University, Taiwan
Education ：Ph. D. Graduate Program in Microbiology and Molecular Genetics, Rutgers University, The State University of New Jersey, New Brunswick, , New Jersey, U.S.A.
Specialties：Cell Biology, Cancer Biology, Immunology
TEL：(03)211-8800 ext. 3721
Dr. Huang’s research is focused on the interactions between viruses and stem cells. Stem cells residing in tissues are thought to be responsible for the repair mechanism in various organs. Recent reports have showed that stem cells can be infected by pathogens such as bacteria and viruses and with pathological sequelae. Her lab has identified several stem cell lines which were especially semsitive to specific virus attack. The primary objective of her studies is to examine the effects of viral infection on target stem cells and elucidate the possible pathological mechanisms.
Huang HI*, Chio CC, Lin JY. Inhibition of EV71 by curcumin in intestinal epithelial cells. PLoS One, 2018 Jan 25;13(1)
Huang HI*, Chang YY, Lin JY, Kuo RL, Liu HP, Shih SR, Wu CC. Interactome analysis of the EV71 5' untranslated region in differentiated neuronal cells SH-SY5Y and regulatory role of FBP3 in viral replication. Proteomics, 2016, Sep. 16; (17):2351-62.
Huang HI*, Shih SR. Neurotropic Enterovirus Infections in the Central Nervous System. Viruses, 2015 Nov 24;7(11):6051-66.
Weng KF, Hsieg PT, Huang HI, Shih SR. Mammalian RNA virus-derived small RNA: biogenesis and functional activity. Microbes Infect. 2015 Aug;17(8):557-63.
Kuo RL, Lin YH, Wang RY, Hsu CW, Chiu YT, Huang HI, Kao LT, Yu JS, Shih SR, Wu CC. Proteomics analysis of EV71-infected cells reveals the involvement of host protein NEDD4L in EV71 replication. J Proteome Res. 2015 Apr 3;14(4):1818-3
Weng KF, Hung CT, Hsieh PT, Li ML, Chen GW, Kung YA, Huang PN, Kuo RL, Chen LL, Lin JY, Wang RY, Chen SJ, Tang P, Horng JT, Huang HI, Wang JR, Ojcius DM, Brewer G, Shih SR. A cytoplasmic RNA virus generates functional viral small RNAs and regulates viral IRES activity in mammalian cells. Nucleic Acids Res. 2014 Nov 10;42(20):12789-805.
Huang HI, Lin JY, Yeh SB, Kuo RL, Weng KF, Shih SR. Enterovirus 71 infects brain-derived neural progenitor cells. Virology. 2014 Oct 6;468-470C:592-600.
Huang HI*, Chen SK, Wang RY, Shen CR, Cheng YC. Human foreskin fibroblast-like stromal cells can differentiate into functional hepatocytic cells. Cell Biol Int. 2013 Dec; 37(12):1308-19.
Wang RY, Kuo RL, Ma WC, Huang HI, Yu JS, Yen SM, Huang CR, Shih SR. Heat shock protein-90-beta facilitates enterovirus 71 viral particles assembly. Virology. 2013 Sep 1;443(2):236-47.
Chen CC, Huang HI*, Louis CL, Lin KT, Ron Y. Isolation of multilineage progenitors from mouse brain. In Vitro Cell Dev Biol Anim. 2013 May;49(5):307-14.
Huang HI*, Chen SK, Ling QD, Chien CC, Liu HT, Chan SH. Multilineage Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin. Tissue Engineering (in press)(*corresponding author) (SCI, Impact factor: 4.699)
Chung CA, Lin TH, Chen SD, Huang HI. Hybrid cellular automaton modeling of nutrient modulated cell growth in tissue engineering constructs. Journal of Theoretical Biology, 2009, 262(2):267-278. (SCI, Impact factor: 2.454)
Yen BL, Chien CC, Chen YC, JT Chen, Huang JS, Lee FK, Huang HI*. Placenta-derived multipotent cells differentiate into neuronal and glial cells in vitro. Tissue Engineering 2008, 14(1):9-17. (*corresponding author) (SCI, Impact factor: 4.699)
Yen ML, Chien CC, Chiu IM, Huang HI, Chen YC, Hu HI, Yen BL. Multilineage differentiation and characterization of the human fetal osteoblastic 1.19 cell line: a possible in vitro model of human mesenchymal progenitors. Stem Cells. 2007 Jan;25(1):125-31. (SCI) (IF: 7.741)
Chang CJ, Yen ML, Chen YC, Chien CC, Huang HI, Bai CH, Yen BL. 2006, Placenta-derived multipotent cells exhibit immunosuppressive properties that are enhanced in the presence of interferon-gamma. Stem Cells. 24(11):2466-77. (SCI) (IF: 7.741)
Chien CC, Fu WH, Huang HI, Lai YH, Tsai YF, Guo SL, Wu TJ, Ling QD. 2006, Expression of Neurotrophic Factors in Neonatal Rats After Peripheral Inflammation. J Pain. (SCI, Impact factor: 3.387)
Chien CC, Yen BL, Lee FK, Lai TH, Chen YC, Chan SH, Huang HI*. 2006, In vitro differentiation of human placenta-derived multipotent cells into hepatocyte-like cells. Stem Cells 24(7): 1759-1768. (*corresponding author) (SCI) (IF: 7.741)
Yen BL, Huang HI, Chih-Cheng Chien, Hsiang-Yiang Jui, Ming Yao, Bor-Sheng Ko, Chia-Tung Shun, Men-Luh Yen, Meng-Chou Lee, Yao-Chang Chen Isolation of Multipotent Cells from Human Term Placenta. Stem Cells. 2005, 23(1):1-6. (Contribution equally) (SCI) (IF: 7.741)
Huang HI, Wu PY, Teo CY, Chen MN, Chen YC, Silin D, Tao MH. 2004, Dec. Improved immunogenicity of a self tumor antigen by covalent linkage to CD40 ligand. Int J Cancer. 108(5):696-703. (SCI) (IF: 4.737)
Chen HW, Huang HI, Lee YP, Chen LL, Liu HK, Cheng ML, Tsai JP, Tao MH, Ting CC. 2002, Aug. Linkage of CD40 to a self-tumor antigen enhances the antitumor immune responses of dendritic cell based treatment. Cancer Immunol Immunother. 51(6):341-8. (SCI) (IF:3.804)
Huang TH, Wu PY, Lee CN, Huang HI, Hsieh SL, Kung J, Tao MH. 2000, Dec. Enhanced antitumor immunity by fusion of CTLA-4 to a self tumor antigen. Blood 96(12):3663-70. (SCI) (IF: 10.432)
Chan MM, Huang HI, Fenton MR, Fong D. 1998, Jun, In vivo inhibition of nitric oxide synthase gene expression by curcumin, a cancer preventive natural product with anti-inflammatory properties. Biochem Pharmacol. 55(12):1955-62. (SCI) (IF:4.838)
Chan MM, Fong D, Ho CT, Haung HI. 1997, Dec, Inhibition of inducible nitric oxide synthase gene expression and enzyme activity by epigallocatechin gallate, a natural product from green tea. Biochem Pharmacol. 55(12):1281-6. (SCI) (IF:4.838)
Chan MM, Ho CT, Huang HI. 1995, Effects of three dietary phytochemicals from tea, rosemary and turmeric on inflammation-induced nitrite production. Cancer Lett. 4;96(1):23-9. (SCI) (IF:4.838)
1.In Vitro Hepatic Differentiation of Multipotent Cells Derived from Human Placenta and Umbilical Cord. Hsing-I Huang, Tsung-Hsuan Lai, B Linju Yen, Yao-Chang Chen, Shu-Hui Chan, Chih-Cheng Chien. 5th ISSCR Symposium, Cairns, AU. 2007.
2.Expression of hepatocyte-like phenotypes in human placenta-derived multipotent cells after HGF and FGF-4 induction. Hsing-I Huang, Tsung-Hsuan Lai, Fa-Kung Lee, B Linju Yen, Yao-Chang Chen, Shu-Hui Chan, Chih-Cheng Chien.
3.國際幹細胞研討會, Taipei, Taiwan, 2006.
4.Differential Maturation of Human Monocyte-derived Dendritic Cells by Lipopolysaccharide and poly I:C. Li-Chiu Su , and Hsing-I Huang*.台灣分子醫學研討會Taipei, Taiwan, 2006.
5.Isolation of Multipotent Progenitors from Human Term Umbilical Cord. Shu-Hui Chan, Tsung-Hsuan Lai and Hsing-I Huang*.台灣分子醫學研討會Taipei, Taiwan, 2006.
1.Huang HI*. Isolation of Placenta-Derived Multipotent Cells from Human Placenta and In Vitro Differentiation into Hepatocyte-Like Cells. Current Protocols in Stem Cell Biology. Unit 1H.1, Wiley Interscience.
2.Chan MM, Huang HI, Mattiacci JA, Fong D. 2002, Jul, Modulation of cytokine gene expression by curcumin. In “Food factors for disease prevention and health promotion” edited by Shahidi F, Ho CT, and Watanabe S. American Chemical Society Press, Washington DC.
2. 胎盤幹細胞及其製備方法。陳耀昌,顏伶汝,簡志誠,黃幸宜 (I307717)
3. Y.C. Chen, L.J. Yen, C.C. Chien, H.I. Huang “Multipotent Stem Cell and Methods Thereof” U.S. Patent Pending, 2004. (60/535502)