李國銘助理教授 Dr. Kuo-Ming Lee
Phone: 886-3-2118800 ext.3727
Fax: 886-3-2118700
E-mail: g39002007@mail.cgu.edu.tw
Education
Ph.D. Institute of Molecular Medicine, College of Medicine, National Taiwan University.
M.S., Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University.
B.S., Department of Life Sciences, National Tsing Hua University.
Research experience
Feb. 2021-present
Assistant Professor, Research Center for Emerging Viral Infections, Chang Gung University.
Aug. 2017-Jan. 2021
Assistant Research Fellow, Research Center for Emerging Viral Infections, Chang Gung University.
Aug. 2015-Jul. 2017
Postdoctoral fellow, Research Center for Emerging Viral Infections, Chang Gung University
Aug. 2010-Jul. 2015
Postdoctoral Fellow, Institute of Biomedical Sciences, Academia Sinica
Research interests
- Study of the host-virus interactions of RNA viruses including enterovirus, influenza virus and coronavirus.
- Identification of undefined pathogens of patients with infections of unknown etiology.
- Investigation of the viral mRNP-remodeling during the viral replication cycle.
- Characterization of the transcription/replication-coupled RNA processing and the mechanism of RNA surveillance.
Technical skills
- Biological chemistry and molecular biology
- Cell biology
- Techniques in epidemiology and molecular virology
Awards:
- National Science Council undergraduate monographic study support (NSC 89-2815-C-001-032R-B), 2000.
- 2010 First Research Competition (Travel Award for International Conference) of Institute of Biomedical Sciences, Academia Sinica.
- 2013 First Research Competition (Travel Award for International Conference) of Institute of Biomedical Sciences, Academia Sinica.
- Poster presentation award at the 2014 Academia Sinica Mini Symposium on RNA Biology, Academia Sinica.
Publications (* corresponding): https://scholar.google.com.tw/citations?user=oBcQRd8AAAAJ&hl=zh-TW
- Sheng-Yu Huang, Yu-An Kung, Peng-Nien Huang, Sheng-Yun Chang, Yu-Nong Gong, Yi-Ju Han, Huan-Jung Chiang, Kuan-Ting Liu, Kuo-Ming Lee, Chia-Yu Chang, Chia-Ching Chang, Chung-Guei Huang, Shin-Ru Shih* (2021 Mar). Stability of SARS-CoV-2 Spike G614 Variant Surpasses That of the D614 Variant after Cold Storage. mSphere, 6(2), e00104-21 (SCIE: 4.282, 33/136, Microbiology).
- Yu-Nong Gong, Kuo-Chien Tsao, Mei-Jen Hsiao, Chung-Guei Huang, Peng-Nien Huang, Po-Wei Huang, Kuo-Ming Lee, Yi-Chun Liu, Shu-Li Yang, Rei-Lin Kuo, Kuan-Fu Chen, Yen-Chin Liu, Sheng-Yu Huang, Hsing-I Huang, Ming-Tsan Liu, Ji-Rong Yang, Cheng-Hsun Chiu, Cheng-Ta Yang, Guang-Wu Chen, Shin-Ru Shih* (2020 Dec). SARS-CoV-2 genomic surveillance in Taiwan revealed novel ORF8-deletion mutant and clade possibly associated with infections in Middle East. Emerging Microbes & Infections, 9(1), 1457-1466 (SCIE: 5.776, 21/136, Microbiology).
- Chung-Guei Huang#, Kuo-Ming Lee#, Mei-Jen Hsiao, Shu-Li Yang, Peng-Nien Huang, Yu-Nong Gong, Tzu-Hsuan Hsieh, Po-Wei Huang, Ya-Jhu Lin, Yi-Chun Liu, Kuo-Chien Tsao, Shin-Ru Shih* (2020, Jul). Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19. Journal of Clinical Microbiology, 58(8), e01068-20 (SCIE: 5.897, 20/136, Microbiology). # equal contribution.
- Bo-Shiun Chen, Hou-Chen Lee, Kuo-Ming Lee, Yu-Nong Gong and Shin-Ru Shih* (2020, Feb). Enterovirus and Encephalitis. Frontiers in Microbiology, 11, 261 (SCIE: 4.236, 34/136, Microbiology).
- Kuo-Ming Lee, Chih-Ching Wu, Shang-En Wu, Ya-Han Lin, Li-Ting Wang, Chun-Ru Chang, Peng-Nien Huang, Shin-Ru Shih, Rei-Lin Kuo* (2020, Apr). The RNA-dependent RNA polymerase of enterovirus A71 associates with ribosomal proteins and positively regulates protein translation. RNA Biology, 17(4), 608-622 (SCIE: 5.350, 48/297, Biochemistry & Molecular Biology).
- Kuo-Ming Lee, Yu-Nong Gong, Shin-Ru Shih* (2020, Nov). Methods for detection and study of virus-derived small RNAs produced from the intramolecular base-pairing region of the picornavirus genome. Methods, 183,4-12 (SCIE: 3.812, 17/77, Biochemical Research Methods).
- Tzu-Wei Chuang, Chia-Chen Lu, Chun-Hao Su, Pei-Yu Wu, Sarasvathi Easwvaran, Chi-Chieh Lee, Hung-Che Kuo, Kuan-Yang Hung, Kuo-Ming Lee, Ching-Yen Tsai, Woan-Yuh Tarn* (2019, Mar). The RNA Processing Factor Y14 Participates in DNA Damage Response and Repair. iScience, 13, 402-415 (SCIE: 4.447, 15/71, Multidisciplinary Sciences).
- Andrew Woodman*, Kuo-Ming Lee, Richard Janissen, Yu-Nong Gong, Nynke H. Dekker, Shin-Ru Shih, Craig E. Cameron* (2019, Feb). Predicting Intraserotypic Recombination in Enterovirus 71. Journal of Virology, 93(4), e02057-18 (SCIE: 4.501, 8/37, Virology).
- Kuo-Ming Lee, Yu-Nong Gong, Tzu-Hsuan Hsieh, Andrew Woodman, Nynke H. Dekker, Craig E. Cameron, Shin-Ru Shih* (2018, Jun). Discovery of Enterovirus A71-like nonstructural genomes in recent circulating viruses of the Enterovirus A species. Emerging Microbes & Infections, 7(1),111 (SCIE: 5.776, 21/136, Microbiology).
- Kuo-Ming Lee, Chi-Jene Chen, and Shin-Ru Shih* (2017, Jul). Regulation Mechanisms of Viral IRES-Driven Translation. Trends in Microbiology, 25(7), 546-561 (SCIE: 13.546 , 8/297, Biochemistry & Molecular Biology).
- Chuan-Tien Hung, Yu-An Kung, Mei-Ling Li, Gary Brewer, Kuo-Ming Lee, Shih-Tung Liu*, and Shin-Ru Shih* (2016, Oct). Additive Promotion of Viral Internal Ribosome Entry Site-Mediated Translation by Far Upstream Element-Binding Protein 1 and an Enterovirus 71-Induced Cleavage Product. PLoS Pathogen, 12(10), e1005959 (SCIE: 6.218, 3/37, Virology).
- Rei-Lin Kuo*, Zong-Hua Li, Li-Hsin Li, Kuo-Ming Lee, Ee-Hong Tam, Helene M. Liu, Hao-Ping Liu, Shin-Ru Shih, and Chih-Ching Wu* (2016, May). Interactome Analysis of the NS1 Protein Encoded by Influenza A H1N1 Virus Reveals a Positive Regulatory Role of Host Protein PRP19 in Viral Replication. Journal of Proteome Research, 15(5),1639-48 (SCIE: 4.074, 12/77, Biochemical research methods).
- Tzu-Wei Chuang, Kuo-Ming Lee, Yuan-Chao Lou, Chia-Chen Lu and Woan-Yuh Tarn* (2016, Apr). A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. Journal of Biological Chemistry, 291(16), 8565-74 (SCIE: 4.238, 87/297, Biochemistry & Molecular Biology).
- Tzu-Wei Chuang#, Kou-Ming Lee#, and Woan-Yuh Tarn* (2015, Apr). Function and Pathological Implications of Exon Junction Complex Factor Y14. Biomolecules, 5(2), 343-55 (SCIE: 4.082, 98/297, Biochemistry & Molecular Biology). # equal contribution.
- Kuo-Ming Lee and Woan-Yuh Tarn* (2014, Nov). TRAP150 activates splicing in composite terminal exons. Nucleic Acids Research, 42(20),12822-32 (SCIE: 11.502, 15/297, Biochemistry & Molecular Biology).
- Kuo-Ming Lee and Woan-Yuh Tarn* (2013, Mar). Coupling pre-mRNA processing to transcription on the RNA factory assembly line. RNA Biology, 10(3), 380-90 (SCIE: 5.350, 48/297, Biochemistry & Molecular Biology).
- Tzu-Wei Chuang, Wei-Lun Chang, Kuo-Ming Lee, and Woan-Yuh Tarn* (2013, Jan). The RNA-binding protein Y14 inhibits mRNA decapping and modulates processing body formation. Molecular Biology of the Cell, 24(1), 1-13 (SCIE: 3.791, 89/195, Cell Biology).
- Kuo-Ming Lee, Ia-Wen Hsu and Woan-Yuh Tarn* (2010, Jun). TRAP150 activates pre-mRNA splicing and promotes nuclear mRNA degradation. Nucleic Acids Research, 38(10), 3340-50 (SCIE: 11.502, 15/297, Biochemistry & Molecular Biology).
- Yi-Tzang Tsai, Chen-I Lin, Hung-Kai Chen, Kuo-Ming Lee, Chia-Yi Hsu, Shun-Jen Yang, and Ning-Hsing Yeh* (2008, Jul). Chromatin tethering effects of hNopp140 are involved in the spatial organization of nucleolus and the rRNA gene transcription. Journal of Biomedical Science, 15(4), 471-86 (SCIE: 5.762, 17/139, Medicine, Research, & Experimental).
- Wen-Cheng Chang, Yung-Chia Chen, Kuo-Ming Lee and Woan-Yuh Tarn* (2007, Mar). Alternative splicing and bioinformatic analysis of human U12-type introns. Nucleic Acids Research, 35(6), 1833-41 (SCIE: 11.502, 15/297, Biochemistry & Molecular Biology).
Database (* corresponding)
Lee, K.-M.,