Current contact: Tel: +886-3-2118800 ext. 3726
E-mail: s0006627c@hotmail.com

Education
2013- 2019.
Graduate Institute of Biomedical Sciences, Division of Biotechnology, Chang Gung University, Tao-Yuan, Taiwan.
2012-2013 M.S.
Graduate Institute of Medical Biotechnology, Chang Gung University, Tao-Yuan, Taiwan.
2008-2012 B.S.
Department of Biomedical Sciences, Chang Gung University, Tao-Yuan, Taiwan.

Professional Experience
2011/06
College Student Research Training Fellowship, National Science Council
2020/1- until now NIH, USA (Project manager) Title: United World Antiviral Research Network (UWARN)

Honors & Awards
2015: American Society for Virology (ASV meeting) Student Travel Award. U.S.A.
2016: American Society for Virology (ASV meeting) Student Travel Award. U.S.A.
2017: Excellent Paper Award of the Second Joint Symposium of Taiwan Virus Society
2018: Chang Gung University Institute of Biomedical Research, Wall Paper Competition, 2nd place
2019: Excellent Paper Award of Taiwan Virus Society Annual Conference & Symposium
2019: Professor Yan Zhizhong Scholarship from Taiwan Society of Microbiology

Patent
1.
Huang SY, Chen CJ, Shih SR “METHOD OF TREATING INFLUENZA A” US Patent granted, 2017(US 9,556,436 B2).
2.
Lin LT, Hsieh EF, Chang CK, Huang SY, Shih SR “hSLU7 transgenic cells for promoting influenza virus replication” 中華民國專利, 2019 (發明第 I 640628號).
3.
施信如 (TW) SHIH, SHIN-RU;龔俞安 (TW) KUNG, YU-AN;江歡容 CHIANG, HUAN-JUNG;洪傳典 (TW) HUNG, CHUAN-TIEN;龔于農 (TW) GONG, YU-NONG;邱馨平 (TW) CHIU, HSIN-PING;黃瓊瑰 (TW) HUANG, CHIUNG-GUEI;黃鵬年 (TW) HUANG, PENG-NIEN;黃聖愉 (TW) HUANG, SHENG-YU. “用於抑制病毒感染與複製的方法” 中華民國專利, 2022 (發明第 I 766709號).

Publications
1.Chen YH, Chang GK, Kuo SM, Huang SY, Hu IC, Lo YL,*Shih SR. Well-tolerated Spirulina extract inhibits influenza virus replication and reduces virus-induced mortality. Sci Rep.2016 Apr 12;6:24253. doi: 10.1038/srep24253.Kuo

2.SM, Chen CJ, Chang SC, Liu TJ, Chen YS, Huang SY, *Shih SR. Inhibition of avian influenza A virus replication in human cells by host restriction factor TUFM is correlated with autophagy. mBio, 2017. pii: e00481-17. doi: 10.1128/mBio.00481-17.

3.Chen MF, Weng KF, Huang SY, Liu YC , Tseng SN ,David M. , *Shih SR . Pretreatment with a heat-killed probiotic modulates monocyte chemoattractant protein-1 and reduces thepathogenicity of influenza and enterovirus 71 infections. Mucosal Immunol. 2017 Jan;10(1):215-227.

4.Huang SY, Huang CH, Chen CJ, Chen TW, Lin CY, Lin YT, Kuo, SM, Huang CG, Lee LA, Chen YH, Chen MF, Kuo RL and *Shih SR. Novel role of miR-1290 in host species specificity of influenza A virus. Mol Ther Nucleic Acids. 2019 May 15;17:10-23. doi: 10.1016/j.omtn.2019.04.028.

5.Hui-Chen Hung , Yi-Yu Ke, Sheng Yu Huang, Peng-Nien Huang , Yu-An Kung , Teng-Yuan Chang, Kuei-Jung Yen, Tzu-Ting Peng, Shao-En Chang, Chin-Ting Huang , Ya-Ru Tsai , Szu-Huei Wu , Shiow-Ju Lee, Jiunn-Horng Lin, Bing-Sin Liu, Wang-Chou Sun , Shin-Ru Shih , Chiung-Tong Chen, John Tsu-An Hsu. Discovery of M Protease inhibitors encoded by SARS-CoV-2. Antimicrob Agents Chemother. 2020 Jul 15;AAC.00872-20.

6.Yu-Nong Gong, Kuo-Chien Tsao, Mei-Jen Hsiao, Chung-Guei Huang, Peng-Nien Huang, Po-Wei Huang, Kuo-Ming Lee, Yi-Chun Liu, Shu-Li Yang , Rei-Lin Kuo, Kuan-Fu Chen, Yen-Chin Liu, Sheng-Yu Huang, Hsing-I Huang, Ming-Tsan Liu, Ji-Rong Yang, Cheng-Hsun Chiu, Cheng-Ta Yang, Guang-Wu Chen, *Shin-Ru Shih. SARS-CoV-2 Genomic Surveillance in Taiwan Revealed Novel ORF8-deletion Mutant and Clade Possibly Associated With Infections in Middle East. Emerg Microbes Infect. 2020 Dec;9(1):1457-1466.

7.Sheng-Yu Huang, Yu-An Kung, Peng-Nien Huang, Sheng-Yun Chang, Yu-Nong Gong, Yi-Ju Han, Huan-Jung Chiang, Kuan-Ting Liu, Kuo-Ming Lee, Chia-Yu Chang, Chia-Ching Chang, Chung-Guei Huang, Shin-Ru Shih. Stability of SARS-CoV-2 Spike G614 Variant Surpasses That of the D614 Variant after Cold Storage. mSphere . 2021 Mar 31;6(2):e00104-21.

8.Lik-Voon Kiew, Chia-Yu Chang, Sheng-Yu Huang, Pei-Wen Wang, Choon-Han Heh, Chung-Te Liu, Chia-Hsin Cheng, Yi-Xiang Lu, Yen-Chen Chen, Yi-Xuan Huang, Sheng-Yun Chang, Huei-Yu Tsai, Yu-An Kung, Peng-Nien Huang, Ming-Hua Hsu, Bey-Fen Leo, Yiing-Yee Foo, Chien-Hao Su, Kuo-Chen Hsu, Po-Hsun Huang, Chirk-Jenn Ng, Adeeba Kamarulzaman, Chiun-Jye Yuan, Dar-Bin Shieh, Shin-Ru Shih, Lip-Yong Chung, Chia-Ching Chang. Development of flexible electrochemical impedance spectroscopy-based biosensing platform for rapid screening of SARS-CoV-2 inhibitors. Biosens Bioelectron . 2021 Jul 1;183:113213.

9.Yu-An Kung, Huan-Jung Chiang, Mei-Ling Li, Yu-Nong Gong, Hsin-Ping Chiu, Chuan-tien Hung, Peng-Nien Huang, Sheng-Yu Huang, Pei-Yu Wang, Tsu-An Hsu, Gary Brewer, and Shin-Ru Shih. Acyl-CoA synthetase long-chain family member 4 is involved in viralreplication organelle formation and facilitates virus replication via ferroptosis. mBio . 2022 Jan18;e0271721.

10.Liu KT, Gong YN, Huang CG, Huang PN, Yu KY, Lee HC, Lee SC, Chiang HJ, Kung YA, Lin YT, Hsiao MJ, Huang PW, Huang SY, Wu HT, Wu CC, Kuo RL, Chen KF, Hung CT, Oguntuyo KY, Stevens CS, Kowdle S, Chiu HP, Lee B, Chen GW, Shih SR.Quantifying Neutralizing Antibodies in Patients with COVID-19 by a Two-Variable Generalized Additive Model.mSphere. 2022 Feb 2:e0088321.

11.Yu-An Kung, Kuo-Ming Lee, Huan-Jung Chiang, Sheng-Yu Huang, Chung-Jung Wu, Shin-Ru Shih. Molecular Virology of SARS-CoV-2 and Related Coronaviruses. Microbiol Mol Biol Rev. 2022 Mar 28;e0002621.

12.Peng-Jui Wang, Yu-Hao Pang, Sheng-Yu Huang, Jun-Tung Fang, Sui-Yuan Chang, Shin-Ru Shih, Tian-Wei Huang, Yi-Jan Chen & Chi-Kuang Sun. Microwave resonant absorption of SARS-CoV-2 viruses. Sci Rep . 2022 Jul 22;12(1):12596.

13.Yu-An Kung, Sheng-Yu Huang, Chung-Guei Huang, Kuan-Ting Liu, Peng-Nien Huang, Kar-Yee Yu, Shu-Li Yang, Chia-Pei Chen, Ching-Yun Cheng, Ing-Kit Lee, Shu-Min Lin, Han-Pin Chang, Yueh-Te Lin, Yen-Chin Liu, Guang-Wu Chen, Shin-Ru Shih. Factors influencing neutralizing antibody titers elicited by coronavirus disease 2019 vaccines. Microbes Infect . 2022 Sep 9;105044

SCIENTIFIC SKILLS
BSL-3 experience(2013-until now) Virology Experiments
Virus amplification and titration by cell lines and egg system
plasmid-based reverse genetics systems
Viral Titer Titration by Plaque Assay and TCID50 Method
Molecular Biology Experiments
Cell Culture and Transient Transfection respiratory primary cell culture and differentiation
Western Immunoblotting
RNA Extraction, RT-PCR, Real-Time PCR
Molecular Cloning
Immunofluorescence Microscopy and Confocal Fluorescence Microscopy
Subcellular Fractionation FLAG Tagged Protein Immunoprecipitation
Luciferase Assay
Primer Extension Assay Northern Blotting Primary Cell Transfection by Electroporation Embryonic Egg Transfection Electroporation Primary Chicken Embryo Fibroblast Cell Culture from Embryonic Egg Generating Stable Cell Lines with Lentivirus Cell viability assay
ELISA
protein or antibody purification
Immunology Experiments
PBMC isolation Flow cytometry ELISPOT
Animal manipulation skills
Intra-nasal
Intraperitoneal injection (IP) In vivo imaging system (IVIS)
RNA therapy (Ferret)
Bioinformatics Tool
BioEdit, DNAstar,Alphafold 2,IVIS imaging, SnapGene, CLCsequence viewer, GraphPad Prism and Image J

TEACHING EXPERIENCE
2013/09-2018/02
General Biology Lectures
2013-now
General laboratory skill training: graduate students
2013-now
Mentor of one under graduate, Master and Ph.D students
CERTIFICATION
2012/12
TOEIC Certification
2013-Now
Manipulation of BSL3 pathogen-- H7N9 and SARS-CoV-2
2020/11/23
The Class Pressure Vessels Personnel (BSL-3 level or above sterilization pressure cooker operation qualification)