湯文芳助理研究員 Dr. Wen-Fang Tang
Expertise: Molecular Biology, Molecular Virology, Cell Biology, and Biochemistry
Wen-Fang Tang obtained her bachelor degree in the department of animal science from National Chung Hsing University and received complete experimental animal operation training. She got her master and Ph.D. degrees in the graduate institute of biomedical science from Chang Gung University. The field of Dr. Tang's research focuses on virus-host interaction by using different technologies to identify host factors involved in EV-A71 replication. She used yeast two-hybrid to screen cellular proteins interact with EV-A71 non-structure proteins and revealed that Reticulon 3 binds with EV-A71 2C protein and is required for viral replication (Tang et al., 2007). She combined miRNA change profiling after EV-A71 infection and proteomic mass-spectrometry-based quantitative proteomics to screen host factors that were modulated by miR-197, whose expression was downregulated by virus infection in a time-dependent manner. She identified that RAN, a miR-197 target gene, supported the nuclear transport of the essential viral proteins 3D/3CD and host protein hnRNP K for viral replication (Tang et al., 2016). Also, she discovered that miR-197 functions as a negative regulator in EV-A71 replication and ETF1 is required for virus replication (Tang et al., 2019). Dr. Tang now focuses on the emerging pathogen animal model establishment, in vivo drug antiviral efficacy test, and using IVIS Spectrum to study virus spread process.