Your browser does not support JavaScript!
郭瑞琳副教授 Dr. Rei-Lin Kuo


Current Appointment: Associate Professor, Department of Medical Biotechnology and Labroatory Sciences, Chang Gung University, Taiwan

Education :國立陽明大學微生物曁免疫研究所博士

Specialties:Molecular virology: Enteroviruses and Influenza viruses

TEL : (03)211-8800 ext. 3775

Mail:rlkuo@mail.cgu.edu.tw

Homepage:

Rei-Lin Kuo received his graduate degrees from the Institute of Microbiology and Immunology at National Yang-Ming University focusing on the encapsidation of spliced RNAs of hepatitis B virus and the molecular mechanism of pathogenesis in Enterovirus type 71 (EV71)-infected cells. After finishing his graduate work, he was trained in influenza virus research at the Institute for Cellular and Molecular Biology at University of Texas at Austin. In 2010, he joined the Research Center for Emerging Viral Infections at Chang Gung University as an assistant professor.
The induction of the Interferon expression is a critical event for innate immunity to disrupt viral replication in virus-infected cells. It has been demonstrated that viruses can evade host innate immunity by targeting the pathway for type I IFN activation. The regulation of type I IFN production has been considered an important factor for virus pathogenicity. Since EV71 is an important human pathogen that periodically causes outbreaks with severe neurological complications in Taiwan, Dr. Kuo’s lab is endeavoring to elucidate the mechanism for the regulation of the signaling pathway for type I IFN production in EV71-infected cells. The understanding of the regulation of IFN production may provide new information concerning the pathogenesis of EV71 infection and the basis for the development of new antiviral drugs.

Publications:

 

Rei-Lin Kuo,* Ya-Han Lin, Robert Yung-Liang Wang, Chia-Wei Hsu, Yi-Ting Chiu, Hsing-I Huang, Li-Ting Kao, Jau-Song Yu, Shin-Ru Shih, and Chih-Ching Wu.* Proteomics analysis of EV71-infected cells reveals the involvement of host protein NEDD4L in EV71 replication. 2015. Journal of Proteome Research, 14:1818-1830. (*co-corresponding author)

Kin Fong Lei,* Chia-Hao Huang, Rei-Lin Kuo,* Cheng-Kai Chang, Kuan-Fu Chenf, Kuo-Chien Tsao, and Ngan-Ming Tsang. Paper-based enzyme-free immunoassay for rapid detection and subtyping of influenza A H1N1 and H3N2 viruses. 2015. Analytica Chimica Acta, 883:37–44. (*co-corresponding author)

Rei-Lin Kuo, Shu-Li Yang, Yi-Chun Liu, Lu-Ting Chen, Chee-Keng Mok, Shu-Ming Kuo, Shin-Ru Shih, and Kuo-Chien Tsao. Influenza A/B virus detection and influenza A virus subtyping with emphasis on the novel H7N9 virus by using multiplex real-time RT-PCR. 2014. Journal of Virological Methods, 208:41-46

Yen-Chin Liu, Rei-Lin Kuo, Jing-Yi Lin, Peng-Nien Huang, Yi Huang, Hsuan Liu, Jamine J. Arnold, Shu-Jen Chen, Robert Yung-Liang Wang, Craig E. Cameron, Shin-Ru Shih. Cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering the nucleus and interfering with Prp8. 2014. PLoS Pathogens, 10(6):e1004199

Chih-Heng Huang, Chi-Jene Chen, Chia-Tsui Yen, Cheng-Ping Yu, Peng-Nien Huang, Rei-Lin Kuo, Sue-Jane Lin, Cheng-Kai Chang, and Shin-Ru Shih. Caspase-1 deficient mice are more susceptible to influenza A virus infection with PA variation. 2013. Journal of Infectious Diseases, 208(11):1898-905

Robert Y.L. Wang, Rei-Lin Kuo, Wei-Chieh Ma, Hsing-I Huang, Jau-Song Yu, Sih-Min Ye, Chi-Ruei Huang, Shin-Ru Shih. Heat shock protein-90-beta facilitates enterovirus 71 viral particles assembly. 2013. Virology, 443(2):236-247

Rei-Lin Kuo*, Li-Ting Kao, Sue-Jane Lin, Robert YL. Wang, and Shin-Ru Shih. MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation. 2013. PLoS One, 8(5):e63431. (*corresponding author)

Rei-Lin Kuo and Shin-Ru Shih. Strategies to develop antivirals against enterovirus 71. 2013. Virology Journal, 10:28. (Highly accessed article)

Qiaozhen Ye, Tom S. Y. Guu, Douglas A. Mata, Rei-Lin Kuo, Bartram Smith, Robert M. Krug, and Yizhi J. Tao. Biochemical and structural Evidence in support of a coherent model for the formation of the double-helical influenza A virus ribonucleoprotein. 2013. mBio, 4(1):e00467-12

Rei-Lin Kuo, Chen Zhao, Meghana Malur, and Robert M. Krug. The NS1 proteins of different influenza A virus subtypes employ strikingly different strategies counter the host antiviral response. 2010. Virology, 408(2):146-158

Chen Zhao, Tien-Ying Hsiang, Rei-Lin Kuo, and Robert M. Krug. ISG15 conjugation system targets the viral NS1 protein in influenza A virus-infected cells. 2010. Proceedings of the National Academy of Sciences of the USA, 107(5), 2253-2258

Chen Zhao, Rei-Lin Kuo, Robert M. Krug. Chapter 1: The NS1 Protein of Influenza A Virus. 2010. Influenza: Molecular Virology. Caister Academic Press. Publication date: February 2010

Rei-Lin Kuo and Robert M. Krug. 2009. The influenza virus polymerase is an integral component of the NS1A:CPSF30 complex in infected cells. Journal of Virology, 83:1611-1616

Laura L. Newcomb*, Rei-Lin Kuo*, Qiaozhen Ye, Yizhi Jane Tao, and Robert M. Krug. 2009. Interaction of the influenza A virus nucleocapsid protein with the viral RNA polymerase potentiates unprimed viral RNA replication. Journal of Virology, 83:29-36 (*equal contribution)

Kalyan Das, Li-Chung Ma, Rong Xiao, Brian Radvansky, James Aramini, Li, Zhao, Jesper Marklund, Rei-Lin Kuo, Karen Y. Twu, Eddy Arnold, Robert M. Krug, Gaetano T. Montelione. 2008. Structural basis for suppression by influenza A virus of a host antiviral response. Proceedings of the National Academy of Sciences of the USA, 105:13093-13098

Karen Y. Twu*, Rei-Lin Kuo*, Jesper Marklund, and Robert M. Krug. 2007. The H5N1 influenza virus NS genes selected after 1998 enhance virus replication in mammalian cells. Journal of Virology, 81:8112-21 (*equal contribution)

Karen Y. Twu, Diana L. Noah, Ping Rao, Rei-Lin Kuo, and Robert M. Krug. 2006. The CPSF30 Binding Site on the NS1A Protein of Influenza A Virus Is a Potential Antiviral Target. Journal of Virology, 80:3957-3965

Shin-Ru Shih, Yih-Weng Wang, Guang-Wu Chen, Luan-Yin Chang, Tzou-Yien Lin, Mei-Chen Tseng, Chiayn Chiang, Kuo-Chien Tsao, Chung Guei Huang, Mei-Ren Shio, Jui-Hung Tai, Shin-Hwan Wang, Rei-Lin Kuo, and Wu-Tse Liu. 2003. Serotype-specific detection of enterovirus 71 in clinical specimens by DNA microchip array. Journal of Virological Methods, 111:55-60

Rei-Lin Kuo, Szu-Hao Kung, Yueh-Ying Hsu, and Wu-Tse Liu. 2002. Infection enterovirus 71 or expression of its 2A protease induces apoptotic cell death. Journal of General Virology, 83:1367-1376

Wu-Tse Liu, Jun-Ren Sun, Chi-Hung Lin, Rei-Lin Kuo, and Szu-Hao Kung. 2001. An indicator cell assay for detection of human cytomegalovirus based on enhanced green fluorescent protein. Journal of Virological Methods, 96:85-92

Szu-Hao Kung, Yu-Chun Wang, Chi-Hung Lin, Rei-Lin Kuo, and Wu-Tse Liu. 2000. Rapid diagnosis and quantification of herpes simplex virus with a green fluorescent protein reporter system. Journal of Virological Methods, 90:205-12

 

Conference presentations:

Kuo, RL., Yang, SL., Liu, YC., Chen, LT., Mok, CK., Kuo SM., Shih, SR., and Tsao, KC. Differential Detection of Avian H7N9 Influenza A Virus Using Highly Sensitive Multiplex Real-time RT-PCR. American Society for Virology 33rd Annual Meeting. June 21-25, 2014, Fort Collins, Colorado, USA

Kuo, RL., Kao, LT., Lin, SJ., Wang RY., and Shih, SR. MDA5 plays an important role in enterovirus 71 RNA-mediated IRF3 activation. American Society for Virology 32nd Annual Meeting. July 19-24, 2013, State College, Pennsylvania, USA

Kuo, RL., Hsieh, EF., Lin, LT., Chiang, C, Chang, YC., Chang CK., Chen GW., and Shih, SR. Differential roles of SLU7 on the expression of M2 ion channel protein of influenza A virus in human versus avian cells. XVth International Congress of Virology. Sept 11-16, 2011, Sapporo, Japan

Kuo, RL., Zhao, C., Malur, M., and Krug, RM. Whether interferon- transcription is activated in influenza A virus-infected cells depends on the source of the viral NS1. American Society for Virology 29th Annual Meeting. July 17-21, 2010, Bozeman, Montana, USA

Kuo, RL., Marklund, J., Twu, KY., and Krug, RM. The influenza A virus polymerase binds to and stabilizes the complex of the NS1A protein with CPSF30 that mediates the inhibition of IFN mRNA production. American Society for Virology 27th Annual Meeting. July 12-16, 2008, Ithaca, New York, USA

Kuo, RL., Twu, KY., Marklund, J., and Krug, RM. The H5N1 influenza virus NS genes selected after 1998 enhance virus replication in mammalian cells. American Society for Virology 26th Annual Meeting. July 14-18, 2007, Corvallis, Oregon, USA

Newcomb, L., Kuo, RL., Ye, Q., Tao, J., and Krug, RM. Influenza viral nucleocapsid protein directs the influenza viral RNA dependent RNA polymerase towards viral replication in vitro. Thirteenth International Conference--Negative Strand RNA Viruses. June 17-22, 2006, Salamanca, Spain

Kuo, RL., Kung, SH., Hsu, YY., Yeh, TC., and Liu, WT. Mechanism of apoptosis in enterovirus 71 infection. XIIth International Congress of Virology. July 27-Aug 1, 2002, Paris, France

Kuo, RL., Tsai, CH., Chaou, WJ., Tsao, LY., Lin, WH., Shin, MC., and Liu, WT. Comparative study on two strains of enterovirus 71 isolated from 1998 outbreak in Taiwan. XIth International Congress of Virology. Aug 16-20, 1999, Sydney, Australia

 

Click Num  
  • Print
  • Add to my favorie .
  • Share
Forward to friend
Please input CAPTCHA
Voice Play
refresh captcha