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李國銘助理研究員 Dr. Kuo-Ming Lee
李國銘助理研究員
 

Kuo-Ming Lee (李國銘)

 

Assistant Research Fellow

Research Center for Emerging Viral Infections

Chang Gung University

259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan, 333

Phone: 886-3-2118800 ext.3723

Fax: 886-3-2118700

E-mail: g39002007@ym.edu.tw

 

Education

09/1997-07/2001

B.S., Department of Life Sciences, National Tsing Hua University.

09/2001-07/2003

M.S., Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University.

09/2005-07/2010

Ph.D. Institute of Molecular Medicine, College of Medicine, National Taiwan University.

 

Postdoctoral Training

08/2010-07/2015

Institute of Biomedical Sciences, Academia Sinica.

08/2015-present

Research Center for Emerging Viral Infections, Chang Gung University.

 

Research interests

1.   Study of the virus-host cell interactions involved in the replication control of picornaviruses.

2.   Investigation of the remodeling of the post-splicing mRNP and the control of nucleocytoplasmic transport.

3.   Characterization of the transcription-coupled pre-mRNA processing and the mechanism of nuclear RNA surveillance.

 

Research experiences

08/2015-present: Postdoctoral fellow with Dr. Shin-Ru Shih, working as the participant of the Human Frontier Science Program (HFSP RGP0011/2015 with the title: Stabilizing RNA virus vaccine strains by elucidating triggers and mechanism of recombination).

07/2010-07/2015: Postdoctoral fellow with Dr. Woan-Yuh Tarn, characterizing the mechanism of splicing regulation mediated by TRAP150.

07/2005-06/2010: Graduate student under the supervision of Dr. Woan-Yuh Tarn, working on characterization of the roles of TRAP150 in nuclear pre-mRNA processing.

10/2003-06/2005: Research assistant under the direction of Dr. Woan-Yuh Tarn, studying on characterization of the phosphorylation status of RBM4 on its nucleocytoplasmic shuttling property.

07/2001-06/2003: Graduate Student under the direction of Dr. Ning-Hsing Yeh, studying on identification of self-self interacting domains and associated proteins of hNopp140.

07/1999-02/2001: Undergraduate student under the guidance of Dr. Woan-Yuh Tarn, studying on identification of the transport cargoes carried by transportin-SR2.

 

Technical skills

1.   Techniques in biological chemistry and molecular biology: isotope handling; DNA and RNA manipulation and gene cloning; PCR and RT-PCR experiment; rapid amplification of cDNA 3' ends (3’RACE) assay; yeast-two hybrid screening assay; recombinant protein expression and purification; protein pull-down and immunoprecipitation assay; nuclear and cytoplasmic extract preparation; in vitro and in vivo kinase assay; in vitro transcription; in vitro translation assay; in vitro and in vivo splicing assay; glycerol gradient sedimentation analysis; Western, Southern, and Northern blot analysis; RNase protection assay; electrophoretic mobility shift assay (EMSA).

2.   Techniques in cell biology: maintenance of cell lines; transient transfection assay; establishment of the stable cell line; metabolic labeling of the cells; indirect immunofluorescence staining; heterokaryon assay.

3.   Techniques in epidemiology and molecular virology: sequence retrieval and analysis; phylogenetic and recombination analysis; handling of infectious viruses; virus amplification and plaque assay; growth curve examination.

4.   Animal experiemnt: Xenopus oocytes manipulation and microinjection.

 

Meetings attended

1.   Poster presentation at the 16th Symposium on Recent Advances in Cellular and Molecular Biology, Pingtung, Taiwan, 2008;

2.   Poster presentation at the 3rd RNA Symposium, National Cheng Kung University, Tainan, Taiwan, 2010;

3.   Poster presentation at the 18th Symposium on Recent Advances in Cellular and Molecular Biology, Pingtung, Taiwan, 2010;

4.   Poster presentation at the 2013 Eukaryotic mRNA Processing meeting, Cold Spring Harbor Laboratory, U. S. A., 2013;

5.   Oral presentation at the 2013 Academia Sinica-NHRI Mini Symposium on RNA Biology, National Health Research Institutes, Miaoli, Taiwan, 2013;

6.   Poster presentation at the 2014 Academia Sinica Mini Symposium on RNA Biology, Academia Sinica, Taipei, Taiwan, 2014;

7.   Poster presentation at the 35th Annual Meeting for the American Society for Virology, Virginia Tech, U. S. A., 2016.

 

Awards:

1.   National Science Council undergraduate monographic study support (NSC 89-2815-C-001-032R-B), 2000.

2.   2010 First Research Competition (Travel Award for International Conference) of Institute of Biomedical Sciences, Academia Sinica.

3.   2013 First Research Competition (Travel Award for International Conference) of Institute of Biomedical Sciences, Academia Sinica.

4.   Poster presentation award at the 2014 Academia Sinica Mini Symposium on RNA Biology, Academia Sinica.

 

Publications (* corresponding)

1.   Lee, K.-M., Shih, S.-R.*, Extensive genomic recombination among the viruses of enterovirus A species. Manuscript in preparation.

2.   Lee, K.-M., Chen, C.-J., Shih, S.-R.* (2017) Regulation Mechanisms of Viral IRES-Driven Translation. Trends Microbiol. 2017, doi: 10.1016/j.tim.2017.01.010. [Epub ahead of print].

3.   Hung, C.-T., Kung, Y.-A., Li, M.-L., Brewer, G., Lee, K.-M., Liu, S.-T.*, Shih, S.R.* (2016) Additive Promotion of Viral Internal Ribosome Entry Site-Mediated Translation by Far Upstream Element-Binding Protein 1 and an Enterovirus 71-Induced Cleavage Product. PLoS Pathog. 2016, 12:e1005959.

4.   Kuo, R.-L.*, Li, Z.-H., Li, L.-H., Lee, K.-M., Tam, E.-H., Liu, H.-M., Liu, H.-P., Shih, S.-R., Wu, C.-C.* (2016) Interactome Analysis of the NS1 Protein Encoded by Influenza A H1N1 Virus Reveals a Positive Regulatory Role of Host Protein PRP19 in Viral Replication. J. Proteome Res. 2016, 15:1639-48.

5.   Chuang, T.-W., Lee, K.-M., Lou, Y.-C., Lu C.-C., Tarn, W.-Y.* (2016) A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. J. Biol. Chem. 2016, 291:8565-74.

6.   Chuang, T.-W., Lee, K.-M.#, Tarn, W.-Y.* (2015) Function and Pathological Implications of Exon Junction Complex Factor Y14. Biomolecules. 2015, 5:343-55.
# equal contribution

7.   Lee, K.-M., Tarn, W.-Y.* (2014) TRAP150 activates splicing in composite terminal exons. Nucleic Acids Res. 2014, 42:12822-32.

8.   Lee, K.-M., Tarn, W.-Y.* (2013) Coupling pre-mRNA processing to transcription on the RNA factory assembly line. RNA Biol. 2013, 10:380-90.

9.   Chuang, T.-W., Chang, W.-L., Lee, K.-M., Tarn, W.-Y.* (2013) The RNA-binding protein Y14 inhibits mRNA decapping and modulates processing body formation. Mol. Biol. Cell. 2013, 24:1-13.

10.     Lee, K.-M., Hsu, Ia-W., Tarn, W.-Y.* (2010) TRAP150 activates pre-mRNA splicing and promotes nuclear mRNA degradation. Nucleic Acids Res. 2010, 38:3340-50.

11.     Tsai, Y.-T., Lin, C.-I., Chen, H.-K., Lee, K.-M., Hsu, C.-Y., Yang, S.-J., Yeh, N.-H.* (2008) Chromatin tethering effects of hNopp140 are involved in the spatial organization of nucleolus and the rRNA gene transcription. J. Biomed. Sci. 2008, 15:471-86.

12.     Chang, W.-C., Chen, Y.-C., Lee, K.-M., Tarn, W.-Y.* (2007) Alternative splicing and bioinformatic analysis of human U12-type introns. Nucleic Acids Res. 2007, 35:1833-41.

 

Database (* corresponding)

Lee, K.-M., Tarn, W.-Y.* (2012) THRAP3/TRAP150 in Atlas of Genetics and Cytogenetics in Oncology and Haematology (a structured review; http://atlasgeneticsoncology.org/Genes/THRAP3ID42960ch1p34.html).

 

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